WASHINGTON, DC - AUGUST 21:  Sen. Elizabeth Warren (D-MA) speaks at the National Press Club August 21, 2018 in Washington, DC. Warren spoke on ending corruption in the nation's capital during her remarks. (Photo by Win McNamee/Getty Images)
Win McNamee/Getty Images
WASHINGTON, DC - AUGUST 21: Sen. Elizabeth Warren (D-MA) speaks at the National Press Club August 21, 2018 in Washington, DC. Warren spoke on ending corruption in the nation's capital during her remarks. (Photo by Win McNamee/Getty Images)
Now playing
02:18
Cherokee Nation: Unhelpful to cling to DNA
Trump in Georgia on October 15, 2018.
POOL
Trump in Georgia on October 15, 2018.
Now playing
00:34
Trump responds to Warren's DNA results
WASHINGTON, DC - MARCH 22:  U.S. Sen. Elizabeth Warren (D-MA) speaks during a rally in front of the Capitol March 22, 2017 in Washington, DC. Sanders urged the U.S. Sen. Bernie Sanders (I-VT) to reject President Donald Trump's nomination of Jay Clayton to head the Securities and Exchange Commission.  (Photo by Alex Wong/Getty Images)
Alex Wong/Getty Images
WASHINGTON, DC - MARCH 22: U.S. Sen. Elizabeth Warren (D-MA) speaks during a rally in front of the Capitol March 22, 2017 in Washington, DC. Sanders urged the U.S. Sen. Bernie Sanders (I-VT) to reject President Donald Trump's nomination of Jay Clayton to head the Securities and Exchange Commission. (Photo by Alex Wong/Getty Images)
Now playing
01:51
Elizabeth Warren releases DNA test results
US Senator Elizabeth Warren (C) greets demonstrators protesting US Supreme Court nominee Brett Kavanaugh on October 4, 2018, in Washington, DC. - A new FBI investigation into Kavanaugh found nothing to corroborate sexual assault allegations against US President Donald Trump's nominee for the Supreme Court, US Senator Chuck Grassley of Iowa said Thursday. (Photo by Jim WATSON / AFP)        (Photo credit should read JIM WATSON/AFP/Getty Images)
Jim Watson/AFP/Getty Images
US Senator Elizabeth Warren (C) greets demonstrators protesting US Supreme Court nominee Brett Kavanaugh on October 4, 2018, in Washington, DC. - A new FBI investigation into Kavanaugh found nothing to corroborate sexual assault allegations against US President Donald Trump's nominee for the Supreme Court, US Senator Chuck Grassley of Iowa said Thursday. (Photo by Jim WATSON / AFP) (Photo credit should read JIM WATSON/AFP/Getty Images)
Now playing
00:35
Sen. Warren will take 'hard look' at 2020 run
President Donald Trump, right, meets with Navajo Code Talkers Peter MacDonald, center, and Thomas Begay, left, in the Oval Office of the White House in Washington, Monday, Nov. 27, 2017. (AP Photo/Susan Walsh
Susan Walsh/AP
President Donald Trump, right, meets with Navajo Code Talkers Peter MacDonald, center, and Thomas Begay, left, in the Oval Office of the White House in Washington, Monday, Nov. 27, 2017. (AP Photo/Susan Walsh
Now playing
01:05
Trump makes Pocahontas crack to code talkers
Cynthia Nixon @ 15:33:52:28  Elizabeth Warren @ 15:58:05:17
CNN
Cynthia Nixon @ 15:33:52:28 Elizabeth Warren @ 15:58:05:17
Now playing
01:54
Warren: Trump an expert in political division
CNN
Now playing
02:02
White House: 'Pocahontas' not racial slur
WASHINGTON, DC - APRIL 07:  U.S. Sen. Elizabeth Warren (D-MA) waits for the beginning of a hearing before the Senate Banking, Housing and Urban Affairs Committee April 7, 2016 on Capitol Hill in Washington, DC. The committee held a hearing on "The Consumer Financial Protection Bureau's Semi-Annual Report to Congress."  (Photo by Alex Wong/Getty Images)
Alex Wong/Getty Images North America/Getty Images
WASHINGTON, DC - APRIL 07: U.S. Sen. Elizabeth Warren (D-MA) waits for the beginning of a hearing before the Senate Banking, Housing and Urban Affairs Committee April 7, 2016 on Capitol Hill in Washington, DC. The committee held a hearing on "The Consumer Financial Protection Bureau's Semi-Annual Report to Congress." (Photo by Alex Wong/Getty Images)
Now playing
01:39
Who is Elizabeth Warren?
Getty Images
Now playing
01:21
Mutual dislike between Trump, Sen. Warren
Brandon Ancil/CNN
Now playing
00:56
Warren: Trump gets 'F' on draining the swamp
pool
Now playing
00:48
Trump slams Warren during NRA speech
SEN WARREN- IT'S CLEAR IT'S TIME FOR SPECIAL PROSECUTOR
CNN
SEN WARREN- IT'S CLEAR IT'S TIME FOR SPECIAL PROSECUTOR
Now playing
01:14
Warren: No doubt Comey firing is a cover-up
WASHINGTON, D.C. - FEBRUARY 8: (AFP-OUT) U.S. President Donald Trump speaks to the Major Cities Chiefs Association (MCCA) Winter Meeting on February 8, 2017 in Washington, DC. During his speech, Trump said one of the main reasons he was elected was his stance on law and order. (Photo by Chris Kleponis-Pool/Getty Images)
Pool/Getty Images North America/Getty Images
WASHINGTON, D.C. - FEBRUARY 8: (AFP-OUT) U.S. President Donald Trump speaks to the Major Cities Chiefs Association (MCCA) Winter Meeting on February 8, 2017 in Washington, DC. During his speech, Trump said one of the main reasons he was elected was his stance on law and order. (Photo by Chris Kleponis-Pool/Getty Images)
Now playing
01:26
Trump refers to Warren as 'Pocahontas' again
warren tears up immigration ban sot _00003726.jpg
warren tears up immigration ban sot _00003726.jpg
Now playing
00:56
Sen. Warren: Overturn Trump's executive order
AFP/Getty Images
Now playing
01:07
Elizabeth Warren: Trump a 'thin-skinned, racist bully'
(CNN) —  

President Donald Trump revived Tuesday his “Pocahontas” nickname for Elizabeth Warren, a day after the Massachusetts senator released a DNA test amplifying the controversy over her claimed Native American heritage.

“Pocahontas (the bad version), sometimes referred to as Elizabeth Warren, is getting slammed,” Trump tweeted of his potential 2020 challenger.

“She took a bogus DNA test and it showed that she may be 1/1024, far less than the average American. Now Cherokee Nation denies her, “DNA test is useless.” Even they don’t want her. Phony!” Trump said.

Pocahontas was a historical figure from the 17th Century and using her name in an intentionally disparaging way insults native peoples and degrades their cultures. The largest Native American advocacy group has said that is why it has condemned the President’s usage in this manner.

In a second tweet moments later, Trump alleged that Warren’s claims of Native American heritage “have turned out to be a scam and a lie” and called for Warren to apologize for the second time in 24 hours.

Warren first faced scrutiny for her purported Native American heritage during her 2012 Senate race.

The results

02:18 - Source: CNN
Cherokee Nation: Unhelpful to cling to DNA

But Warren now has documentation to back up her family lore – an analysis of her genetic data performed by Carlos Bustamante, a professor of genetics at Stanford and adviser to Ancestry and 23 and Me.

Bustamante’s full report is now posted on Warren’s website, along with other supporting documents and interviews detailing her background.

According to the report, “the great majority of (Warren’s) identifiable ancestry is European.” However, the report adds, “The analysis also identified 5 genetic segments as Native American in origin at high confidence.”

Bustamante’s analysis places Warren’s Native American ancestor between six and 10 generations ago, with the report estimating eight generations.

“The identity of the sample donor, Elizabeth Warren, was not known to the analyst during the time the work was performed,” the report said.

Warren released the findings of the test in a video in which she concedes that she is not enrolled in any tribe, “but my family history is my family history.”

What counts as Native American heritage is a question with different answers among the 573 legally recognized tribes in the United States.

Cherokee Nation Secretary of State Chuck Hoskin Jr. released a statement Monday saying using a DNA test to claim a connection to any tribal nation is “inappropriate and wrong.”

“It makes a mockery out of DNA tests and its legitimate uses while also dishonoring legitimate tribal governments and their citizens, whose ancestors are well documented and whose heritage is proven,” he said. “Senator Warren is undermining tribal interests with her continued claims of tribal heritage.”

Trump thanked the Cherokee Nation on Twitter Tuesday for “revealing that Elizabeth Warren, sometimes referred to as Pocahontas, is a complete and total Fraud!”

Trump’s $1 million challenge

Trump’s nickname for Warren is among his most frequently used monikers. The President most notoriously referred to Warren as Pocahontas at an official White House event honoring veteran Navajo code talkers.

On Monday Trump claimed that he “didn’t say” that he would pay $1 million to Democratic Sen. Elizabeth Warren for taking DNA test to review her Native American heritage.

“Who cares?” Trump initially said when first asked about the DNA test. When pressed on the once-promised $1 million payment, Trump responded: “I didn’t say that. You better read it again.”

In fact, Trump did promise $1 million, during a July rally, but only if the test showed she was “an Indian.”

At a rally in July, Trump said: “And we will say, ‘I will give you a million dollars, paid for by Trump, to your favorite charity if you take the test and it shows you’re an Indian … we’ll see what she does. I have a feeling she will say no but we will hold it for the debates.”

Later Monday, Trump said he did not owe Warren the money, saying that was contingent on her winning the nomination.

“If she gets the nomination in the debate where I was going to have her tested,” Trump said.

“I’ll only do it if I can test her personally,” he said. “This is not something I would enjoy doing either.”

CNN’s Dan Merica contributed to this report.