(CNN)Donald Trump is at it again.
As Fox News host Megyn Kelly returned from vacation, the Republican presidential candidate used Twitter late Monday night to slam her return during the airing of her show, saying that host "must have had a terrible vacation, she is really off her game."
Then he tweeted another one, suggesting Kelly goes back on vacation: "I liked The Kelly File much better without @megynkelly. Perhaps she could take another eleven day unscheduled vacation!"
He even retweeted a tweet calling her a "bimbo" and another that says "she came back looking like Nancy Grace."
This is the second time that Trump retweeted a message that refers to Kelly as a "bimbo," an act for which he was sharply criticized the first time.
Trump and Fox News president Roger Ailes had reportedly cleared the air after the first wave of the fighting began, but by Tuesday afternoon, the channel executive released a statement, signaling the detente was over.
"Donald Trump's surprise and unprovoked attack on Megyn Kelly during her show last night is as unacceptable as it is disturbing," Ailes said in a statement obtained by CNNMoney, adding "Donald Trump rarely apologizes, although in this case, he should."
Trump's first Twitter rant slamming Kelly came the night of the first presidential debate, after Kelly asked him about misogynistic, sexist comments he made in the past, such as calling some women "fat pigs, dogs, slobs, and disgusting animals," to which, he responded with "only Rosie O'Donnell."
This comes after the real estate mogul was under fire for escalating a feud with Kelly when he told CNN's Don Lemon earlier this month that Kelly "had blood coming out of her eyes, blood coming out of her wherever."
Trump drew swift condemnation from conservatives and was uninvited from the RedState Gathering, a conservative event featuring GOP presidential hopefuls earlier this month in Atlanta.