(CNN) -- Chelsea have been rocked by a leg injury to England left-back Ashley Cole, which threatens to keep him out of action for several weeks.
Cole has been diagnosed with a depressed fracture of the tibia after aggravating the problem prior to Sunday's 1-0 home victory over Manchester United.
The defender missed three games after initially sustaining the injury in the 4-0 home win against Atletico Madrid in the Champions League last month.
He made his comeback against the same side in the return Group D game last week but required a painkilling injection in order to play against United.
A spokesman for Chelsea told Press Association Sport: "Ashley Cole has not been selected in the England squad for their friendly against Brazil.
"He has a depressed fracture of the tibia, an injury first picked up in the Atletico Madrid home match last month.
"He aggravated the injury before the match against Manchester United and required an injection to play.
"However, he further injured it during the game and has therefore been ruled out of this week's England squad."