(CNN) -- World No. 1 Roger Federer held off the challenge of Argentine Jose Acasuso to reach the third round of the Cincinnati Masters, where he will be joined by defending champion Andy Murray and Rafael Nadal -- but not two-time champion Andy Roddick.
Federer held off Jose Acasuso to continue his progress in the Cincinnati Masters.
The top-seeded Swiss went through 6-3 7-5 in one hour and 10 minutes to earn a clash with David Ferrer.
Acasuso was broken in the eighth game, with Federer -- who was beaten in the quarterfinals of the Montreal Masters -- then serving out to take the opening set.
The 28-year-old French Open and Wimbledon champion broke again in the 11th game of the second set to secure victory.
Federer, who like all the top seeds had an opening-round bye, told Sky Sports: "I am very happy to have made the transition from my Montreal defeat."
On unseeded Spaniard Ferrer, he added: "He is one of the best return players in the game. I will have to be careful even though I have a good record against him."
Fifth seed Roddick was upset 7-6 (13-11) 7-6 (7-3) by fellow American Sam Querrey in a near two-hour battle.
Querrey earned revenge for his defeat by Roddick in the third round of the Legg Mason Classic in Washington which ended his run of reaching three successive ATP Tour finals.
The 21-year-old, who lost in the first round in Montreal last week, will next face former world No. 1 and two-time Cincinnati finalist Lleyton Hewitt, who defeated Benjamin Becker 6-3 6-3.
New world No. 2 Murray, who climbed above Nadal en route to winning the Montreal Masters, will face Czech 16th seed Radek Stepanek in the third round after winning his opening game 7-6 (7-3) 6-2 unseeded Spaniard Nicolas Almagro -- his third-round conqueror at the French Open.
Stepanek battled back to win 4-6 6-3 6-1 against Marat Safin to mark the former world No. 1's final appearance at the tournament ahead of his retirement at the end of this year.
Nadal, seeded second ahead of Murray, continued his rehabilitation from long-term knee problems with a 7-6 (7-4) 7-6 (7-3) victory over unseeded Italian.
The Spaniard, who lost his No. 1 ranking after missing Wimbledon, will next play Paul-Henri Mathieu following the unseeded Frenchman's 7-6 (11-9) 6-4 victory over big-serving Croatian Ivo Karlovic.
Fourth seed Novak Djokovic was first on Center Court on Wednesday, and the Serbian prevailed in a close contest against Croatian Ivan Ljubicic.
The pair were closely matched with Djokovic taking the first set 7-5 on the tie-break before he won the second set 6-4 after crucially converting one of his four break points.
Seventh seed Jo-Wilfried Tsonga, who upset Federer in Montreal, was the day's other major casualty, going down 7-6 6-2 to Australian qualifier Chris Guccione.