(CNN) -- Former England captain Michael Vaughan has been left out of the 16-strong training squad which will prepare for the Ashes series against Australia that starts next month.
Vaughan has failed to impress with his form for county side Yorkshire this season.
It is a bitter blow for the 34-year-old, who led England to a memorable Ashes triumph on home soil in 2005, but has struggled since with injury and loss of form.
Vaughan has not played for England since resigning the captaincy last August and was hoping to impress the selectors with his performances with county side Yorkshire.
But he has yet to score a century in the county championship this season and has had a recurrence of problems to his right knee.
Vaughan has not played for England since resigning the captaincy last August. He has recently had a recurrence of problems to his right knee.
He has lost out to Ian Bell who will offer the main challenge to the current incumbents in the middle order batting for a spot in the starting team.
National selector Geoff Miller said he knew Vaughan would be "disappointed" not to be included.
"He understands that he needs to make runs consistently in county cricket if he is to force his way back into the squad," he added.
There is also no place for another 2005 hero, fast bowler Steve Harmison, who has been taking wickets for his county side Durham but has failed to win a recall.
Instead the selectors have opted to go for his Durham teammate Graham Onions, Tim Bresnan and Ryan Sidebottom as back-up to first-choice pacemen James Anderson and Stuart Broad.
Key all-rounder Andrew Flintoff is in the training party having proved his fitness after a string of injury problems, while all three of the spinners are in contention for a Test place with Monty Panesar, Graeme Swann and Adil Rashid given the chance to prove themselves.
The wicket at Cardiff, where the first Test of the five-Test series is being staged, is believed to offer help to slower bowlers and England may well go in with two spinners.
England are coming off a 2-0 series win over West Indies, but failed to progress from the Super Eight phase of the World Twenty20 Cup.